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11 Mtodos de deteccin Mtodo de titulacin Procedimiento de anlisis: Pesar aproximadamente 0.1 g de la muestra secada hasta peso constante (precisin de 0.0001 g), aadir 20 mL de cido actico glacial y luego realizar una titulacin potenciomtrica con una solucin valorante estndar de cido perclrico (0.1 mol/L)
[1] This medication works by enhancing insulin secretion in response to meals, suppressing glucagon release, slowing gastric emptying, and reducing appetite
On the day of assay, the mannitol and sucrose (MAS) buffer (1) was prepared by diluting sterile-filtered MAS 3 in distilled water, resulting in a solution with the following final concentrations: 220 mM mannitol, 70 mM sucrose, 10 mM KH 2 PO 4 , 5 mM MgCl 2 , 2 mM HEPES, 1 mM EGTA and 0.2% w/v fatty-acid free BSA (3117057001) (pH 7.2), and warmed to 37 C
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