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however, because the patient was not taking hormonal contraception at the time of her visit to our department, this therapy was contraindicated

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However, some individuals may find that allowing it to reach room temperature for about 30 minutes reduces discomfort during injection

Peptides were separated using an Acclaim Pepmap 100 trap column (75 micron ID 2 cm from Themo Scientific) and eluted onto an Acclaim PepMap RSKC analytical column (75 micron ID x 2 cm, Thermo Scientific) with a gradient of solvent A (water and 0.1% formic acid) and solvent B (acetonitrile and 0.1% formic acid)