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Post-translational modification analysis For MS analysis of PDHc-E2 or OGDHc-E2 modifications, immunoprecipitation eluates were separated on a pre-cast 4% to 12% NuPAGE gel (Thermo Fisher Scientific, NP0321BOX) and protein bands were stained using SimplyBlue SafeStain solution (Thermo Fisher Scientific, LC6060)
Among these 154 drugs, 96 are single-target GLP1R drugs, while dual-target combinations include 19 for GIPR x GLP1R, 16 for GCGR x GLP1R, 7 for INSR x GLP1R, 3 for FGF21R x GLP1R, 2 for GLP-2R x GLP1R, and 1 for FGF21 x GLP1R