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Biological replicates, isotope-labeled internal standards, and repeated QC checks are used to separate true biological differences from analytical noise, enabling more reliable biological interpretation

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At the same time, liver inflammation, intestinal flora displacement and concurrent infection caused by cirrhosis lead to the release of a large number of inflammatory factors and endotoxemia, causing renal microcirculation dysfunction, which can induce renal function injury (Rad et al., 2024)

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