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If you notice any of the following, the pen should be discarded: Cloudiness or turbidity in the solution Particles, flakes, or crystals floating in the liquid Discolouration of the solution Any signs suggesting the medication has been frozen It's important to note that even if the solution appears normal, this does not guarantee full potency if the pen has been exposed to extreme temperatures

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A vibratome (Leica VT1200S, Leica Biosystems) was used to obtain 250 m thin acute brain slices containing Arc, identified by well-known landmarks