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Donor multilineage reconstitution was assessed by flow cytometry in peripheral blood of recipient mice monthly for 4 months using the antibody mixture consisting of CD45.2, CD45.1, Mac1, Gr1, B220 and CD3 (BioLegend, 109830, 110728, 101208, 108406, 103212 and 100214)

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It is made by boiling water and letting the pure water vapor condense in a new container, leaving anything that is not H 2 O behind

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