They may wish to conduct testing to determine whether theyre possible symptoms of a more serious effect from your medication, such as kidney-related symptoms
Bodipy 665/676 (Thermo Fisher Scientific, B3932, 10 M, 45 min), LysoTracker Deep Red (Thermo Fisher Scientific, L12492, 100 nM, 45 min), DND-189 LysoSensor (Thermo Fisher Scientific, L7535, 100 nM, 1 h), Liperfluo (Dojindo, L248, 1 M, 1 h) and SQSS (in-house, 50 nM, 1 h) were added to live cells before fixation
All treatments are subject to provider review, medical appropriateness, and clinical discretion
A volume of 1 mL complete M199 (Gibco) [supplemented with FBS (10%), penicillin-streptomycin (1%), Fungizone (1%), gentamycin (0.1%) and insulin (1 g/mL)] was placed in each well