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HSCs were spun down at 500 g, 4 C for 10 min, and incubated with Illumina TAGMENT DNA enzyme and buffer (20034210, Illumina) or with tagmentase (Diagenode, C01070012) and the respective buffer (Diagenode C01019043) and Digitonin (0.01%) for 30 min at 37 C on a thermomixer shaking at 700 rpm

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Australian Government Department of Health

Although no adverse pregnancy outcomes were reported in a study of Iranian women who took dehydrated garlic tablets (800 mg/day) for two months during the third trimester of pregnancy (153), the safety of garlic supplements in pregnancy has not been established