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2.10 Overexpression and knockdown of RRM2 gene in TPC-1 and IHH4 cells For upregulating RRM2 expression in cells, we obtained a full-length RRM2 sequence fragment using a PCR method and subsequentlycloned it into vector LV18, verified by DNA sequencing and transfected 293T cells (see Supplementary Materials for details)

4.2.5 Inhibition of ferroptosis in sepsis-induced myocardial injury In sepsis-induced myocardial injury, ferroptosis inhibition may represent a novel therapeutic strategy

Various redox reactions regulate and drive essential processes, such as energy production, biosynthesis, and cellular signaling, through mechanisms collectively referred to as redox regulation [2,3,4,5]

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