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In this study, PNC-27 appeared to exert an inhibitory influence on the proliferation of these primary ovarian cancer cells, alongside an apparent direct cytotoxic action

Absorbance at 490 nm was measured for each well using a microplate reader (Thermo Fisher Scientific, Waltham, MA), with 600 nm as the reference wavelength

Notably, previous studies have confirmed that the aforementioned gut bacteria regulated by dietary fiber (e.g., Bifidobacterium, Lactobacillus, Faecalibacterium ) are closely associated with depression (45), providing a critical link for dietary fiber to intervene in depression via gut microbiota

10.1016/j.tifs.2003.12.004 50