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Cells were then washed twice with Hanks Balanced Salt Solution (HBSS, H8264) and incubated with 200 L GDC (12 concentrations, 05 mM) in DMEM D1145 or 1% TritonX (X-100)

That gap is worth planning around: the beyond-use window of a reconstituted solution (covered in the storage section) can be shorter than the drug supply lasts, especially for the 10 mg vial, so re-order and reconstitution-volume decisions should be made against the shorter of the two limits the stability window or the drug supply not just the drug supply alone

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