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The immunoblotting and immunofluorescence microscopy detection demonstrated that only GSTP-S184D entered the nucleus in LPS-untreated RAW264.7 cells, and LPS triggered GSTP (WT) but not GSTP-S184A to enter the nucleus (Figures 5B,C)

The most defensible synthesis is that the effect of a free synthetic MGF peptide on cultured myoblasts is weak or absent, while E-domain sequences expressed endogenously in vivo do produce measurable, though context- and age-limited, effects two statements that are only superficially contradictory once the difference in experimental design is appreciated

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Structure-activity relationships for the interaction of 5,10-dihydroindeno[1,2-b]indole derivatives with human and bovine carbonic anhydrase isoforms I, II, III, IV, and VI