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Further drawbacks of this study Beyond the citrulline malate confound, remember that Kyowa Hakko funded the study, and one of their employees also designed the experiment, performed the statistical analysis, and assisted in the preparation of the manuscript

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200 g Fmoc-Gly-Wang Resin (21.6 mmol) with a degree of substitution of 0.108 mmol/g was weighed and swelled for 30 min with DMF, followed by sequentially coupling according to the amino acid sequence of Semaglutide with 2-5 equivalents of amino acid feeds and condensing with DIC+A or B+A+C, until the resin was detected to be transparent with ninhydrin, wherein A was HOBt or HOAt