If your downstream purpose doesnt require native, functional antibodies for example, you were just quantifying antibody levels from different conditions then denaturing elution would work just fine for that purpose
In a randomized, double-blind study of 30 CHF patients, all of whom had been taking furosemide (80 mg/day) for at least three months, intravenous (IV) thiamin therapy (200 mg/day) for seven days resulted in an improved LVEF compared to IV placebo (80)
J Alzheimers Dis 59:537541 Shukla D, Mandal PK, Ersland L, Gruner ER, Tripathi M, Raghunathan P, Sharma A, Chaithya GR, Punjabi K, Splaine C (2018) A Multi-center study on human brain glutathione conformation using magnetic resonance spectroscopy
Early intervention can prevent complications and help you get back to enjoying your meals pain-free