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Laboratories [well, NHS labs in the UK] have very sophisticated error-trapping routines

Ice-crystal formation during each freeze-thaw cycle inflicts mechanical damage on molecular structure, and pre-splitting eliminates the cumulative loss that comes from thawing one vial multiple times

Here's the step-by-step process we've refined: Gather Your Supplies: You'll need your vial of lyophilized IGF-1 LR3, a vial of bacteriostatic water, a fresh alcohol prep pad, and a sterile syringe of the appropriate size (typically a 1mL or 3mL syringe)

The single most common error we see is storing opened BAC water on a lab bench between uses