Because of this, the individual contribution of each genetic variant to susceptibility is relatively low.6,7 Vitiligo susceptibility mapping by linkage analysis The mapping of genetic risk factors for vitiligo was performed by linkage analysis followed by positional cloning
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The three libraries were generated using error-prone PCR as described above, using the following conditions to produce varying levels of mutagenesis: Library 1: 2 M 8-oxo-dGTP, 2 M dPTP, 20 PCR cycles Library 2: 4 M 8-oxo-dGTP, 1 M dPTP, 20 PCR cycles Library 3: 8 M 8-oxo-dGTP, 0.5 M dPTP, 20 PCR cycles The library sizes (approximated by number of transformants as described above) were 6 10 7 for each library
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