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The Ube2e2 mutants were generated by two-step PCR and ligated into the pCAG- Maltose-Binding Protein (MBP) or GST vector

Diabetologia (2006) 49:28247

Fibroblasts are the cells responsible for producing collagen, the primary structural protein in tendons

All the cell culture models can be used with a variety of cell types such as immortalized cell lines, primary cultured cells, and pluripotent stem cells