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Metformin may modestly help preserve lean mass through its effects on AMPK activation in muscle tissue, though the evidence for this is preliminary

Untargeted metabolomics analysis by GC/MS For sample analysis, an Agilent 7890B GC system coupled to an electron ionization (EI) mode 5977 A mass selective detector (Agilent Technologies) was used

GPX4 protein was eluted with an elution buffer containing 250 mM imidazole, 1 M NaCl, and 25 mM Tris at pH 8.0

Each meal is built around macrosmeaning the balance of protein, carbs and fatswhich can be especially helpful if youre trying to support workouts or preserve muscle while eating less