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Copper deficiency primarily disrupts lipid homeostasis by impairing the activation of key transcription factors, sterol regulatory element-binding proteins (SREBP-1 and SREBP-2), which regulate fatty acid and cholesterol synthesis [88]

To construct the sorting vector for gene overexpression, we amplified the whole expression cassette of N-signal-GST-EGFP-GPI DAF and N-signal-GST-EGFP-GPI CEAM7 from pEGFP-GST-GPI DAF and pEGFP-GST-GPI CEAM7 vectors and joined them with PCR linearized pcDNA3.1 vector using ClonExpress II One Step Cloning Kit

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Watson et al., 2004