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In summary, melatonin could enhance salt stress signaling by up-regulating the expression of genes related to the synthesis of spermine and spermidine in H

This interference sensitizes tumor cells to chemotherapy and improves treatment efficacy in preclinical cancer models, positioning RP101 as a candidate for combination therapy approaches

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Preparation of protein lysates The cells collected from 6-well plates were lysed in 0.4 ml lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, and 2% SDS) followed by sonication (10 cycles of 10 s pulses with 10 s rest intervals) using a Fisher Scientific Sonic Dismembrator Model 500 at 15% amplitude