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Cells were cultured for 2 h and then read using a ClarioStar-Plus plate reader at absorbances of 570 and 595 nm

In a recent FDA drug safety communication regarding SGLT-2 inhibitor-associated DKA, hypovolemia and acute renal impairment were listed as potential factors that may contribute to the development of a high anion gap metabolic acidosis

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Neil K Gibbs, L-Histidine Supplementation in Adults and Young Children with Atopic Dermatitis (Eczema), The Journal of Nutrition , Volume 150, Issue Supplement_1, October 2020, Pages 2576S2579S, Song X, Qu H, Yang Z, Rong J, Cai W, Zhou H