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This is one of the clinical decisions where I will be firm with the patient and explain why
For determination of the midpoint potentials E of roGFP variants OxD roGFP or, alternatively, the degree of reduction ReD roGFP = 1 OxD roGFP was plotted against the calculated redox potentials of the respective lipoic acid redox buffers and all data points were fitted to a sigmoidal dose-response curve using GraphPadPrism5 (GraphPad Software, www.graphpad.com)
[DOI] [PMC free article] [PubMed] [Google Scholar] Rosario-Cruz Z
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