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The first enzyme, glutamate cysteine ligase (GCL), joins the amino acids glutamate and cysteine together to form gamma-glutamylcysteine (Glyteine)

One of the most significant is body weight, as the dosage of glutathione is often calculated on a weight-based scale, typically ranging from 20 to 40 mg per kilogram per day, divided into 2-3 doses for optimal absorption

However, therapeutic doses for treating deficiency range from high-dose oral supplements (1,0002,000 g daily) to intramuscular injections of 1 mg, particularly for pernicious anaemia or malabsorption conditions