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Arch Biochem Biophys (2025) 768:110366

Effects of chronic crocin treatment on desoxycorticosterone acetate (doca)-salt hypertensive rats

[1] [13] When B12 levels are borderline or clinical suspicion remains high despite normal B12, additional tests include: Holotranscobalamin (active B12) , which may be more sensitive in early or borderline deficiency Methylmalonic acid (MMA) and homocysteine levels, which become elevated in functional B12 deficiency (note that MMA is also elevated in renal impairment, and homocysteine rises in folate and B6 deficiency) Full blood count to identify macrocytic anaemia (elevated mean cell volume) Blood film examination may show hypersegmented neutrophils and megaloblastic changes Serum folate should be checked concurrently, as deficiencies often coexist Investigating the underlying cause is essential for appropriate management

Not every patient achieves the weight loss targets they are hoping for, and a meaningful proportion of patients even on maximum doses do not reach the 1520% body weight reduction that clinical guidelines increasingly use as a benchmark for meaningful metabolic benefit