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The experiments were performed in a buffer solution containing 20 mM HEPES (pH 7.0) and 150 mM NaCl

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The Biological Basis for MOTS-c and 5-Amino-1MQ Synergy MOTS-c is a 16-amino-acid peptide encoded by mitochondrial DNA that functions as a metabolic regulator by activating AMPK (AMP-activated protein kinase)the enzyme that shifts cellular metabolism from anabolic (energy storage) to catabolic (energy utilisation)