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The ELISA kit: Serum levels of E2 (Shanghai Yuanxin, China,YX-030522C), serum levels of FSH (Shanghai Yuanxin, China,YX-061908C), serum levels of LH (Shanghai Yuanxin, China,YX-001208C), serum levels of T(Shanghai Yuanxin, China,YX-202005C), serum levels of PRL (Shanghai Yuanxin, China,YX-161812C), serum levels of P (Shanghai Yuanxin, China,YX-160714C)