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Methods Estrogen deprived MCF-7:2A cells were treated with 1 nM 17-estradiol (E 2 ), 100 M BSO, or 1 nM E 2 + 100 M BSO combination in vitro , and the effects of these agents on cell growth and apoptosis were evaluated by DNA quantitation assay and annexin V and terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) staining

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Cold-Chain Protocols: Specialized handling for sterile injectables