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Each subject received 2 weeks of evening injections and 2 weeks of morning injections

GSH AS A PHYSIOLOGICAL MODULATOR OF NO HOMEOSTASIS The importance of GSH in protecting the electron transport chain from ONOO - is illustrated by experiments with GSH depleted astrocytes

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Lipid peroxidation analysis Cells were harvested with TrypLE Select Enzyme (Thermo Fisher Scientific A1217702) and resuspended in 200 L PBS containing 5 M BODIPY 581/591 C11 (Invitrogen D3861) or 5 M MitoPerOx (Abcam ab146820) for 30 min to measure total lipid peroxidation or mitochondrial lipid peroxidation