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LEfSe analysis provided a secondary measure to establish differences between treatment groups in addition to the one-way ANOVA/KruskalWallis tests performed on each genus/phylum

Photosynthesis of cucumber plants was depressed under the HT treatment based on the light and CO 2 response curves, which could be due to the reduction of chlorophyll content and carotenoid content at high temperatures that impaired the biosynthesis of photosynthetic pigments 33

Interestingly, we found that CPT1 is a downstream protein of AMPK, and inhibition of AMPK expression leads to a decrease in the level of CPT1