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Immunoprecipitation is ideal for analyzing separated protein fractions using other biochemical techniques such as density gradients, sedimentation, or gel filtration

81960253 and 82160268) awarded to BX and YL and Key Laboratory for Research on Autoimmune Diseases of Higher Education Schools in Guizhou Province, (Qianjiaoji [2023] 016) awarded to BX were held by members of our research group during the study period

Mechanism and biology Yoshino, Baur, Imai 2018 (Cell Metabolism) and Covarrubias et al

They proposed that cysteine competitively interferes with PAG binding to the enzyme, thereby preventing PAG from completing the formation of the dead-end complex with Tyr114