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Assessment of oxidative/antioxidative markers in hippocampal tissues Following euthanasia, the hippocampal tissues were immediately excised, weighed, homogenized, and centrifuged at 4000 rpm for 15 min in a buffer containing 137 mM NaCl, 2.7 mM KCl, 10 mM NaHPO, and 2 mM KHPO (pH 7.4)

Research shows that ones metabolism slows down with age