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Immunofluorescence staining of oocytes After a brief rinse with PBS/0.05% polyvinylpyrrolidone (to prevent adhesion), the oocytes were permeabilized with 0.5% Triton X-100/PHEM for 5 min, fixed in 3.7% PFA/PHEM (18.14 g PIPES, 6.5 g HEPES, 3.8 g EGTA, 0.99 g MgSO4, pH 7.0 w/KOH) for 20 min, and washed three times (10 min each) with PBS/polyvinylpyrrolidone

People on GLP-1 medications often struggle with reduced appetite, making food tracking feel like extra mental work

DNA damage-response pathways are some of most important in cancer development, with genomic instability being a key feature of these diseases

Caffeine shows significant chronic psychoactive metabolite accumulation with daily use [34], while paraxanthine and 1-MX have mostly-inactive metabolites that are rapidly excreted [35]