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Add 1,000 L (1 mL) of reconstitution solvent to the 1 mg vial This yields a 1 mg/mL (1,000 g/mL) stock solution To achieve 100 g/mL working stock: Dilute 100 L of 1 mg/mL stock into 900 L of PBS or cell culture medium yields 100 g/mL in 1 mL To achieve 10 ng/mL in a 5 mL experiment: Take 0.5 L of 100 g/mL working stock + 4,999.5 L of medium Alternatively: dilute to an intermediate concentration to reduce pipetting error Use a dilution series approach (stock working stock final experimental concentration) to minimize pipetting inaccuracy at nanogram-level concentrations

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General Guidelines for Female Athletes Dose Range : 1020 micrograms (mcg) per day Frequency : 5 days on, 2 days off (or every other day) Timing : Best: Immediately post-workout with carbs and protein Alternate: First thing in the morning, not fasted Cycle Length : 4 to 6 weeks per cycle Off-Cycle : Equal or greater time off (e.g., 46 weeks) This keeps IGF-1 levels elevated without desensitizing receptors or risking long-term metabolic fatigue