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Ipamorelin is a synthetic pentapeptide that belongs to the growth hormone secretagogue (GHS) class of peptides
The primers used were: 53 F (AAAGCTAGCGCCACCATGAAGACGATCATCGCCCTGAGC) and 53 R (TTTGGCGCGCCCTAA-GAGCAGGACGCCTGACAAGT), ligating the product into pAAV-hSyn-DIO-EGFP (plasmid from Addgene, 50457, RRID: Addgene_50457) in place of the EGFP in NheI and AscI sites to produce the human GLP1R virus construct (AAV-hSyn-DIO-hGLP1R)
Semaglutide is a GLP-1 receptor agonist licensed in the UK for type 2 diabetes mellitus, not weight management
Access to these detailed protocols helps researchers navigate the balance between aggressive weight loss and healthy body composition
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