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The percentage of methemoglobin was determined by colorimetry (Sato, 2005)

One fraction for analysis of GSH-derived metabolites was immediately precipitated with MeOH and after evaporation, obtained residue was resuspended in 50 L of 50 mM HEPES (pH 7.4), diluted with 20% ACN (containing 0.1% FA and mixture of internal standards) and immediately analysed by LC-MS/MS