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Briefly, GSTO1-1 pQE30 plasmid was transformed into M15 cells, which were grown in Luria-Bertani broth supplemented with 100 g ml 1 ampicillin and 25 g ml 1 kanamycin at 37 C to an OD 600 of 0.8 and induced with 0.1 mM isopropyl thio- -D-galactoside for 4 h

The sweet spot for most researchers using a 5mg vial is 1.0mL of bacteriostatic water

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Dietary sources of ALA While ALA supplements provide concentrated doses, certain foods naturally contain ALA, albeit in smaller amounts.54 Incorporating these into your diet can support overall antioxidant intake: Organ meats: Kidney, heart, and liver are among the richest natural sources of ALA