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However, the study was small, lacked a placebo control and evaluated biomarkers rather than long-term clinical outcomes

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According to the results from in vitro and in vivo studies, tanshinone IIA pretreatment protects the liver from APAP-induced liver injury by activating Nrf2 and increasing the mRNA and protein levels of the Nrf2 target genes glutamate-cysteine ligase catalytic subunit (GCLC), NQO1, and HO-1 (Wang et al., 2016)

Additionally, in 2D cultures, ECs can easily spread and migrate due to the lack of physical impediments, while in 3D cultures, a surrounding matrix poses constraints that enforce the ECs adaptation to fit through matrix pores and often require matrix degradation for spreading and migration [240]