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Before any measurement, cells were incubated for one hour with XF Assay Medium (Seahorse Bioscience) plus 5 mM glucose

Retrieved from Wharton-a, S., Aronne, L

Briefly, brain slices were air-dried at 39C for 2 hours, washed with 1 PBS, treated with 3% hydrogen peroxide in methanol and Target Retrieval buffer (Advanced Cell Diagnostics [ACD]) for 15 minutes, and subjected to RNAscope Proteinase III incubation at 40C for 15 minutes

Very often in medicine, observational studies find that two things often happen at the same time for example, countries with high chocolate consumption produce more Nobel prize winners per capita