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Canonical Wnt/-catenin signaling pathway is activated via increased production of Wnt ligands by NTAPP To investigate whether NTAPP exposure affect a biological response via the canonical Wnt/-catenin signaling pathway, we treated hDP cells with two types of pharmacological inhibitor of Wnt/-catenin signaling, endo-IWR1 25 and IWP2 26 before NTAPP treatment

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Add human autologous serum to the medium prepared above to a final concentration of 10%