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Superoxide dismutase, glutathione, malondialdehyde, total antioxidant capacity, and lactate dehydrogenase assays The SOD, MDA, GSH, T-AOC, and LDH levels of dorsal skins and serum were detected by using the SOD, MDA, GSH, T-AOC, and LDH assay kit, respectively, according to the manufacturers instructions (Nanjing Institute of Jiancheng Biological Engineering, China)

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Vitamin B6 is included to further enhance energy metabolism and support the body's ability to combat fatigue