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Animal wound models have confirmed that topical GHK-Cu application reduces neutrophil and macrophage infiltration by approximately 30-50% during the first 48-72 hours post-injury, while maintaining sufficient immune cell presence for bacterial clearance and debris removal

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Varying amounts of monobromobimane stock solution (0-300 l) were added to respective cuvettes already containing 5 l of the enzyme solution (protein concentration of 810 g ml -1 ), glutathione (100 l of stock solution) and phosphate buffer (3.0 ml)

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