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Calculation of Alzheimers disease (AD)-associated microglia signature scores For each single MG transcriptome, the AD-associated MG signature score was calculated using the AddModuleScore function in the Seurat package, as the average normalized expression levels of top 100 upregulated genes in AD-associated MGs 20 subtracted by the aggregated expression of control feature sets 1

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RaTG13 and Khosta-2 S protein-expressing pseudotyped viruses exhibited minimal infectivity in VeroE6/TMPRSS2 cells

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