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RNAse A was added, and samples were incubated at 37 C for 30 min

Absolute contraindications include: Personal or family history of medullary thyroid carcinoma : These medications carry a boxed warning regarding thyroid C-cell tumors based on rodent studies Multiple endocrine neoplasia syndrome type 2 (MEN 2) : Increased risk of medullary thyroid carcinoma Previous serious hypersensitivity reaction : To semaglutide, tirzepatide, or any formulation component Pregnancy considerations: These medications are not recommended during weight management in pregnancy due to limited safety data and potential fetal risks

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