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After Blush regularization, a focus mask that covers the SLC33A1 MAP density but excludes the detergent micelle density, which we refer to as an SLC33A1 MAP TMD focus mask was used to for local refinement of the transmembrane domain, yielding a construction at a resolution of 3.26

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Rossi, R., Dalle-Donne, I., Milzani, A