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For analysis of flavonoids by HPLCMS, reaction volumes were split after the plate reader incubations, and a 40 l aliquot was extracted with 80 l of 75% methanol in H 2 O, 1 mM EDTA, 0.3% hydrochloric acid for quantification of cyanidin ( 8 ) concentration while a second 40 L aliquot was extracted with 80 l of 75% acetonitrile in H 2 O, 1 mM EDTA for quantification of all other flavonoids

They are designed to imitate this hormone, and they stay active in the body much longer than the natural one

While B12 is essential for maternal health and fetal development throughout pregnancy, the third trimester is where the most fetal growth happens