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135,191,192 While this seminal work focused mostly on DNA recognition and the mechanism of transcriptional regulation, these authors noted the presence of a potential metal binding motif, later shown to be critical for imparting metal selectivity ( vide infra )

As the processes of cell replication, nerve function and energy release that use B12 all need a wide range of other nutrients to work properly, it means that nothing will function fully if something is in short supply (it becomes a "limiting factor")

There isn't a one-size-fits-all answer, because the right amount depends entirely on the final concentration your research protocol demands