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[DOI] [PMC free article] [PubMed] [Google Scholar] 94.Hsieh M.J., Liu H.T., Wang C.N., Huang H.Y., Lin Y., Ko Y.S., Wang J.S., Chang V.H., Pang J.S

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Duration of gradual systemic effect on an average individual Fair to medium brown Complexion 1-3 Months Dark Brown Complexion 1-6 Months Very dark Complexion 6-12 months Black Complexion 12-24 months 2

Methods Expression and purification of recombinant OsDHAR Recombinant OsDHAR was expressed in Escherichia coli NiCo21(DE3), purified and crystallized as described previously 30