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The amplitude (A) of spontaneous and Ca 2+ -induced swelling of the organelle suspension in a Ca 2+ -free medium and in the conditions of pore-formation activation with the action of PTP inducer of swelling of calcium ions was calculated as the difference in optical density values from 1 to 15 min mPTP-associated mitochondrial swelling was tested by the specific mPTP inhibitor cyclosporin A (CsA), which was added to the incubation medium in a concentration of 10 5 mol/L 5 min before the induction of pore formation

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For an intramuscular shot that goes deep into the muscle (like in the arm or buttocks), a longer, slightly thicker needle is needed to reach the target