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Although we cannot rule out that the increased production of ROS in 3D culture conditions may not be observed under hypoxia, it should be noted that hypoxia itself induces ROS production in numerous cell types, including tumor cells [39]

Gene centric pathways correlation analyses showed that the glutathione metabolism pathway ( p = 0.001) was highly ranked, as well as its linked taurine and hypotaurine metabolism pathway ( p = 0.009) (Figure 3B and see Supplementary Tables S8)

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