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Although we cannot rule out that the increased production of ROS in 3D culture conditions may not be observed under hypoxia, it should be noted that hypoxia itself induces ROS production in numerous cell types, including tumor cells [39]

Subsequently, the ATP6AP1L coding sequence was amplified from the cDNA of T47D cells and inserted into the FastDigest KpnI/FastDigest XbaI (Thermo Fisher) linearized pcDNA3.1-GST DAF and pcDNA3.1-GST CEAM7 vectors using T4 DNA ligase (EL0011, Thermo Fisher)

Differing ages, injury severity, and injury type may also result in different CBF responses